Journal: The Journal of endocrinology

Article Title: Leptin stimulates aromatase in the growth plate: limiting catch-up growth efficiency.

doi: 10.1530/JOE-18-0028

Figure Lengend Snippet: Fig. 3 (revised version): Immunohistochemistry of aromatase enzyme and the long form of leptin receptor

Article Snippet: Sections were incubated with 145 primary antibodies: 3 different anti-aromatase antibodies (cat. no. MCA2077s, 146 Serotec, Oxford, UK; cat. no. ab18995, Abcam, Cambridge, MA, USA; cat. no. 3599-147 100, Biovision, Milpitas, CA, USA) and anti-leptin receptor (Ob-Rb; cat. no. 148 7 ab177469, Abcam), followed by a biotinylated second antibody and streptavidin-149 peroxidase conjugated with aminoetyl carbazole substrate (Histostain-SP Kit, Zymed 150 Laboratories, South San Francisco, CA, USA).

Techniques: Immunohistochemistry

Journal: The Journal of endocrinology

Article Title: Leptin stimulates aromatase in the growth plate: limiting catch-up growth efficiency.

doi: 10.1530/JOE-18-0028

Figure Lengend Snippet: Fig.4: Effect of leptin on aromatase mRNA expression. ATDC5 cells were cultured in DMEM/F12 containing 5% FCS and ITS for 4 days. (A) Cells incubated with or without (control) 100 ng/ml leptin for 3, 6, 12 and 24 hours. (B) Cells treated with leptin at 0, 10, 100, and

Article Snippet: Sections were incubated with 145 primary antibodies: 3 different anti-aromatase antibodies (cat. no. MCA2077s, 146 Serotec, Oxford, UK; cat. no. ab18995, Abcam, Cambridge, MA, USA; cat. no. 3599-147 100, Biovision, Milpitas, CA, USA) and anti-leptin receptor (Ob-Rb; cat. no. 148 7 ab177469, Abcam), followed by a biotinylated second antibody and streptavidin-149 peroxidase conjugated with aminoetyl carbazole substrate (Histostain-SP Kit, Zymed 150 Laboratories, South San Francisco, CA, USA).

Techniques: Expressing, Cell Culture, Incubation, Control

Journal: The Journal of endocrinology

Article Title: Leptin stimulates aromatase in the growth plate: limiting catch-up growth efficiency.

doi: 10.1530/JOE-18-0028

Figure Lengend Snippet: Fig.6: qPCR analysis and Western blot of aromatase expression in ATDC5 cells. Cells were cultured in DMEM/F12 containing 5% FCS and ITS for 14 days, treated overnight with ITS free medium containing 0.5%

Article Snippet: Sections were incubated with 145 primary antibodies: 3 different anti-aromatase antibodies (cat. no. MCA2077s, 146 Serotec, Oxford, UK; cat. no. ab18995, Abcam, Cambridge, MA, USA; cat. no. 3599-147 100, Biovision, Milpitas, CA, USA) and anti-leptin receptor (Ob-Rb; cat. no. 148 7 ab177469, Abcam), followed by a biotinylated second antibody and streptavidin-149 peroxidase conjugated with aminoetyl carbazole substrate (Histostain-SP Kit, Zymed 150 Laboratories, South San Francisco, CA, USA).

Techniques: Western Blot, Expressing, Cell Culture

Journal: The Journal of endocrinology

Article Title: Leptin stimulates aromatase in the growth plate: limiting catch-up growth efficiency.

doi: 10.1530/JOE-18-0028

Figure Lengend Snippet: Fig.7: Effect of leptin on aromatase promoters PII/PI.3. ATDC5 cells were cultured in DMEM/F12 containing 5% FCS and ITS for 4 days. The cells were transiently

Article Snippet: Sections were incubated with 145 primary antibodies: 3 different anti-aromatase antibodies (cat. no. MCA2077s, 146 Serotec, Oxford, UK; cat. no. ab18995, Abcam, Cambridge, MA, USA; cat. no. 3599-147 100, Biovision, Milpitas, CA, USA) and anti-leptin receptor (Ob-Rb; cat. no. 148 7 ab177469, Abcam), followed by a biotinylated second antibody and streptavidin-149 peroxidase conjugated with aminoetyl carbazole substrate (Histostain-SP Kit, Zymed 150 Laboratories, South San Francisco, CA, USA).

Techniques: Cell Culture

Journal: The Journal of endocrinology

Article Title: Leptin stimulates aromatase in the growth plate: limiting catch-up growth efficiency.

doi: 10.1530/JOE-18-0028

Figure Lengend Snippet: Fig.8: Leptin stimulation of aromatase expression-role of MAPK signaling pathway (ERK1/2). ATDC5 cells were cultured in DMEM/F12 containing 5% FBS and ITS for 4 days. Cells were treated overnight

Article Snippet: Sections were incubated with 145 primary antibodies: 3 different anti-aromatase antibodies (cat. no. MCA2077s, 146 Serotec, Oxford, UK; cat. no. ab18995, Abcam, Cambridge, MA, USA; cat. no. 3599-147 100, Biovision, Milpitas, CA, USA) and anti-leptin receptor (Ob-Rb; cat. no. 148 7 ab177469, Abcam), followed by a biotinylated second antibody and streptavidin-149 peroxidase conjugated with aminoetyl carbazole substrate (Histostain-SP Kit, Zymed 150 Laboratories, South San Francisco, CA, USA).

Techniques: Expressing, Cell Culture

Journal: The Journal of endocrinology

Article Title: Leptin stimulates aromatase in the growth plate: limiting catch-up growth efficiency.

doi: 10.1530/JOE-18-0028

Figure Lengend Snippet: Fig.9: Leptin stimulation of aromatase expression- role of the STAT3 signaling pathways (Tyr 705). ATDC5 cells were cultured in DMEM/F12 containing 5% FCS and ITS for 4 days. Cells were treated overnight with ITS free medium containing 0.5% FCS. (A) Cells were treated with 100 ng/ml of leptin for the indicated

Article Snippet: Sections were incubated with 145 primary antibodies: 3 different anti-aromatase antibodies (cat. no. MCA2077s, 146 Serotec, Oxford, UK; cat. no. ab18995, Abcam, Cambridge, MA, USA; cat. no. 3599-147 100, Biovision, Milpitas, CA, USA) and anti-leptin receptor (Ob-Rb; cat. no. 148 7 ab177469, Abcam), followed by a biotinylated second antibody and streptavidin-149 peroxidase conjugated with aminoetyl carbazole substrate (Histostain-SP Kit, Zymed 150 Laboratories, South San Francisco, CA, USA).

Techniques: Expressing, Protein-Protein interactions, Cell Culture

Journal: The Journal of endocrinology

Article Title: Leptin stimulates aromatase in the growth plate: limiting catch-up growth efficiency.

doi: 10.1530/JOE-18-0028

Figure Lengend Snippet: Fig.10: Leptin stimulation of aromatase expression- role of the PI3K signaling pathway. ATDC5 cells were cultured in DMEM/F12 containing 5% FCS and ITS for 4 days. Cells were treated overnight with ITS free medium containing 0.5% FCS. (A) Cells were treated with 100 ng/ml of leptin for the indicated

Article Snippet: Sections were incubated with 145 primary antibodies: 3 different anti-aromatase antibodies (cat. no. MCA2077s, 146 Serotec, Oxford, UK; cat. no. ab18995, Abcam, Cambridge, MA, USA; cat. no. 3599-147 100, Biovision, Milpitas, CA, USA) and anti-leptin receptor (Ob-Rb; cat. no. 148 7 ab177469, Abcam), followed by a biotinylated second antibody and streptavidin-149 peroxidase conjugated with aminoetyl carbazole substrate (Histostain-SP Kit, Zymed 150 Laboratories, South San Francisco, CA, USA).

Techniques: Expressing, Cell Culture